The Dairy Authority

8215 20th Street, Greeley, CO 80634 (970) 351-8102

TDA Labs - Large Animal Diagnostics

Pregnancy may now be detected at TDA Labs through blood or milk samples. A sample is collected on farm and submitted to the lab. Turn around time is one day or a rush request can be requested for additional cost. Currently the cost per sample is the same across all tests

Summary of pregnancy detection tests at TDA Labs;

BioPRYN-requires blood, 73 days post calving and 28 day post breeding

IDEXX Blood- requires blood, 60 days post calving and 28 day post breeding

IDEXX Milk- requires milk, 60 days post calving and 35 day post breeding

Pregnancy Diagnosis with Blood

BioPRYN is a ruminant-specific pregnancy test developed by Dr. Garth Sasser at the University of Idaho. The test specifically looks for the level of pregnancy-specific protein B (PSPB) in serum. The ELISA reader calculates the optical density of the individual cow and compares this value with known controls including both open and pregnant cows. Based on this comparison, the results are reported as open, pregnant or recheck. These reports can be transferred electronically into on-farm software if the client so chooses. The BioPRYN test requires an overnight incubation period and results are reported approximately 24 hours after the test is first set up.

Animal selection and sample handling- BioPRYN

Cows that were previously pregnant must be at least 73 days postpartum in order for any residual PSPB to be gone. Cows also need to be a minimum of 28 days post insemination. Virgin heifers should be at least 28 days post breeding in order to get an accurate test result. This test only tells you if the cow is pregnant or open. Gestation cannot be staged by this particular assay. Blood should be collected in a clot tube and stored under refrigeration until submission for testing. PSPB is quite stable and there seem to be no untoward effects of serum sitting on the clot. Also, it is not critical that samples be refrigerated for shipment; however, it is a good idea to have them refrigerated prior to shipment. Samples should be shipped for receiving in 1-2 days.

IDEXX blood/milk pregnancy test developed recently by IDEXX specifically looks for pregnancy-associated glycoproteins (PAGs) in serum. The ELISA reader calculates the optical density of the individual cow and compares this value with known controls including both open and pregnant cows. Based on this comparison, the results are reported as open, pregnant or recheck. These reports can be transferred electronically into on-farm software if the client so chooses. The IDEXX tests test requires 4-6 hours to comlete and results are reported approximately 24 hours after the test is first set up.

Animal selection and sample handling- IDEXX

Cows that were previously pregnant must be at least 60 days postpartum in order for any residual PSPB to be gone. Cows also need to be a minimum of 28 days post insemination for blood and at least 35 days post insemination for the milk test. This test only tells you if the cow is pregnant or open. Gestation cannot be staged by this particular assay. Blood should be collected in a clot tube and stored under refrigeration until submission for testing. PAGs are quite stable and there seem to be no untoward effects of serum sitting on the clot. Also, it is not critical that samples be refrigerated for shipment; however, it is a good idea to have them refrigerated prior to shipment. Samples should be shipped for receiving in 1-2 days.

Milk

Just about any milk can be collected for pregnancy detection. Milk from DHIA samples may be used, fresh or frozen milk samples may be used and samples collected by hand pre or post milking may be used. Samples cannot be sour or mastitic.

TDA Labs

TDA Labs has done side by side comparison testing between the BioPRYN tests, IDEXX tests and rectal palpation by experienced DVM palpators. The results of the test have been very comparable with palpation and in most cases are 1-2 % better than palpation results. The lab tests have worked extremely well in virgin heifers and is becoming a preferred means of pregnancy diagnosis for AI bred heifers. In dairy herds, the test can provide the opportunity for more frequent (weekly) checks in some herds where it is difficult for a palpator to get there at the same frequency. This allows for more rapid diagnosis of open cows and an opportunity for earlier re-enrollment into synchronization programs. In addition, the non-invasive nature of the test reduces the chances of embryo damage during early gestational palpation. We would be glad to discuss this test in greater detail with anyone interested in finding out more about it.

For more information in regards to sheep, goats, bison and deer testing, please contact the lab at info@TDALabs.com.

To learn more about delivering samples to the lab, visit the Samples page.

 

Questions may also be submitted through our online contact form.